Synonym ：4-Amino-PGA

4-Aminofolic acid

4-Aminopteroyl-L-glutamic acid

Molecular Formula C19H20N8O5

Molecular Weight 440.41

CAS Number 54-62-6

氨基喋呤 Aminopterin Descriptions

Linkage More potent, but more toxic, than methotrexate.

Biochem/physiol Actions Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn,binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.

Properties

Assay…………………….. ~98% (TLC)

form ………………………...powder

color………………………... yellow

solubility ……………… 2 M NaOH: 50 mg/mL Solutions may be stored for 1-2 days at 4°C.

DMSO: Solutions may be stored for 1-2 days at 4°C.soluble

abs…………………………. ε1M/261 nm, 0.1 M NaOH 25,700

ε1M/282 nm, 0.1 M NaOH 24,500

ε1M/373 nm, 0.1 M NaOH 8,100

storage temp. −20°C