产品说明书:
Thrombin from bovine plasma
Synonym : Factor IIa
CAS Number: 9002-04-4
(EC) Number: 3.4.21.5
凝血酶 Descriptions:
Analysis Note Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot J.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Biochem/physiol Actions Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Preparation Note Activated with bovine brain thromboplastin.
Physical form Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0
凝血酶 Properties:
form ……………………………………………………lyophilized powder
composition …………………………………………Protein, 40-60%
Gene Information ……………………………………cow ... F2(280685)
storage temp…………………………………………−20°C
TEST |
SPECIFICATION |
APPEARANCE |
WHITE TO OFF-WHITE POWDER |
PROTEIN BY BIURET |
APPROX. 50% |
ENZYMATIC ACTIVITY |
40 TO 300NIH UNITS/MG PROTEIN |
UNIT DEFINITION |
ACTIVITY IS EXPRESSED IN NIH UNITS OBTAINED BY DIRECT COMPARISON TO A NIH THROMBIN REFERENCE STANDARD. |
RECOMMENDED RETEST |
6 YEARS |
Leupetin 亮抑酶肽 | 中性蛋白酶 | Xanthione oxidase 黄嘌呤氧化酶 | Trypsin inhibitor 胰蛋白酶抑制剂 | Trypsin 1:250 胰蛋白酶1:250 | Superoxide Dismutase(SOD) 超氧化物歧化酶 |
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