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Thrombin  凝血酶

所属分类:酶和辅酶
商品编号:T4648
商品规格:1000u分装
计量单位:
库存现状:100
参考价:¥450
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详细说明

产品说明书:

Thrombin from bovine plasma

Synonym Factor IIa

CAS Number: 9002-04-4

(EC) Number: 3.4.21.5

凝血酶 Descriptions:

Analysis Note Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot J.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Biochem/physiol Actions Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Preparation Note Activated with bovine brain thromboplastin.

Physical form Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

凝血酶 Properties:

form ……………………………………………………lyophilized powder

composition …………………………………………Protein, 40-60%

Gene Information ……………………………………cow ... F2(280685)

storage temp…………………………………………−20°C

TEST

SPECIFICATION

APPEARANCE

WHITE TO OFF-WHITE POWDER

PROTEIN BY BIURET

APPROX. 50%

ENZYMATIC ACTIVITY

40 TO 300NIH UNITS/MG PROTEIN

UNIT DEFINITION

ACTIVITY IS EXPRESSED IN NIH UNITS OBTAINED BY DIRECT COMPARISON TO A NIH THROMBIN REFERENCE STANDARD.

RECOMMENDED RETEST

6 YEARS

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