• Ribonuclease A from bovine pancreas

Synonym:  Pancreatic Ribonuclease

                     Ribonuclease I

                     Ribonucleate 3’-pyrimidinooligonucleotidohydrolase

                     RNase A

CAS Number:  9001-99-4

(EC) Number:  3.1.27.5  

Descriptions:

Analysis Note:       Protein determined by E1%/280.

Application:           RNase protection assays

Remove unspecifically bound RNA

Analysis of RNA sequences

Hydrolyze RNA contained in protein samples

Purification of DNA

Pancreatic RNase A specifically cleaves at the 3’-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2’-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.

Preparation Note: Salt fractionated and chromatographically purified.

Properties:

Type……………………………………………Type I-A

assay …………………………………≥60% as RNase A (SDS-PAGE)

form ……………………………………………powder

mol wt …………………………………………mol wt ~13,700

total impurities ………………………………salt, essentially free

foreign activity …………………………………protease, essentially free

storage temp………………………………….−20°C

Safety Statements: 22-24/25