产品详细介绍:

 Collagenase from Clostridium histolyticum

release of physiologically active rat epididymal adipocytes tested, Type II, ≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid

Synonym Clostridiopeptidase A

CAS Number 9001-12-1

Enzyme Commission (EC) Number 3.4.24.3

Descriptions

Analysis Note Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.

One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.

One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.

One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.

Properties

suitability release of physiologically active rat epididymal adipocytes tested

storage temp. −20°C