产品的详细说明:

Collagenase from Clostridium histolyticum

for general use, Type I, 0.25-1.0 FALGPA units/mg solid, >125 CDU/mg solid (CDU = collagen digestion units), essentially salt-free, lyophilized powder

Synonym Clostridiopeptidase A

CAS Number 9001-12-1

Descriptions

Preparation Note Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953).

Quality Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.

One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.

One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.

One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.

Properties
Form………………………………….. essentially salt-free, lyophilized powder

storage temp. −20°C