产品展示
点击这里给我发消息
点击这里给我发消息

产品分类

您所在的位置:

AMV Reverse Transcriptase   反转录酶

所属分类:酶和辅酶
商品编号:M5101
商品规格:300u
计量单位:
库存现状:30
参考价:¥630
订购 收藏

商品简介

详细说明

AMV Reverse Transcriptase   反转录酶 Promega M5101  300u

 

Description (反转录酶英文描述)  Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the

polymerization of DNA using template DNA, RNA or RNA:DNA hybrids (1). It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

Features      

Available at High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/μl.

Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2,

2.5mM spermidine, 50mM DTT.

Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C)

Applications      First- and second-strand synthesis of cDNA.

           Primer extensions and RNA sequencing (2).

           RT-PCR. Up to 10μl of an RT reaction containing AMV RT and the supplied AMV

RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase. If GoTaq® DNA Polymerase or PCR Master Mix are used, up to 25μl of an RT reaction can be added per 50μl PCR.

Protocol         Promega Product Information #9PIM510.

Storage Conditions    Store at –20°C.

Storage Buffer   200mM potassium phosphate (pH 7.2 at 4°C), 0.2% Triton® X-100, 2mM DTT and

50% gycerol.

Unit Definition  One unit is defined as the amount of enzyme required to catalyze the transfer of

1nmol of dTTP into acid-insoluble form in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 40mM KCl, 8.75mM MgCl2, 10mM DTT, 0.1mg/ml acetylated BSA, 1mM radiolabeled dTTP and 0.25mM poly(A):oligo(dT).

Quality Control Tests   Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, RT-PCR,

first-strand cDNA synthesis.

Source    Purified Avian Myeloblastosis Virus particles.

References  

1Kacian, D.L. (1977) Meth. Virol. 6, 143.

2Mierendorf, R.C. and Pfeffer, D. (1987) Meth. Enzymol. 152, 563–6.

购买本产品的用户还买过

相关产品

顾客评论

共 页 条记录,当前是第 页
首 上一页 下一页 尾 跳至: