Sigma P4380 Protamine Sulfate 硫酸鱼精蛋白
Protamine sulfate salt from salmon Grade II
Synonym Salmine from salmon
Salmine
CAS Number 53597-25-4
Application May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.
Biochem/physiol Actions Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.
Quality May contain insolubles as well as histones.
Properties
type ……………………………………Grade II
TEST |
SPECIFICATION |
APPEARANCE |
WHITE TO OFF-WHITE POWDER |
SOLUBILITY |
CLEAR COLORLESS TO LIGHT YELLOW SOLUTION AT 25MG/ML IN 6N HYDROCHLORIC ACID (MAY CONTAIN INSOLUBLES) |
GRAVIMETRIC ASSAY |
16 TO 21% SULFATE |
LOSS ON DRYING |
NMT 5% |
ELEMENTAL ANALYSIS |
22 TO 26% NITROGEN |
HISTONES |
NEGATIVE |
SUITABILITY TEST RNA PRECIPITATION
|
NLT 15 UNITS OF RNA PRECIPITATED PER MG PROTAMINE SULFATE |
Transferrin 转铁蛋白 | PHA-P 植物血球凝集素P | Hemoglobin 牛血红蛋白 | Fibrinogen 纤维蛋白原 | Collgen Type I 胶原蛋白I型 | ConA Type A IV 刀豆蛋白A IV |
|
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