产品详细说明:

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

Synonym ： Phaseolus vulgaris agglutinin

PHA

Descriptions:

Analysis Note: Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1hr incubation at 25°C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Biochem/physiol Actions: PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. 1,2

PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. 3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. 4 PHA-M is the mucoprotein form. 4 Conjugates are prepared from the corresponding purified lectins.

Properties:

form …………………………………………………lyophilized powder

potency ……………………………………………<62.5 μg per mL agglutination activity

<10 μg/mL mitogenic activity

total impurities ……………………………………salt, free

storage temp. ………………………………………2-8°C

Safety Statements: 22-24/25